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KMID : 0894520190230030285
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Development & Reproduction
2019 Volume.23 No. 3 p.285 ~ p.295
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A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse
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Lin Zi-Li
Li Ying-Hua
Jin Yong-Xun
Kim Nam-Hyung
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Abstract
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Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assem?bly. The actin-regulatory activity of JMY is based on a cluster of three actin-binding Wiskott-Aldrich syndrome protein ho?mology 2 (WH2) domains that nucleate actin filaments directly and promote nucleation of the Arp2/3 complex. In addition to these activities, we examined the activity of JMY generation in early embryo of mice carrying mutations in the JMY gene by CRISPR/Cas9 mediated genome engineering. We demonstrated that JMY protein shuttled expression between the cytoplasm and the nucleus. Knockout of exon 2, CA (central domain and Arp2/3-binding acidic domain) and NLS-2 (nuclear localization signal domain) on the JMY gene by CRISPR/Cas9 system was effective and markedly impeded embryonic development. Ad?ditionally, it impaired transcription and zygotic genome activation (ZGA)-related genes. These results suggest that JMY acts as a transcription factor, which is essential for the early embryonic development in mice.
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KEYWORD
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Transcription, Mouse, Embryo, Blastocyst
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